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Bloodstream infections are a significant cause of morbidity and mortality in healthcare settings. Accurate and timely diagnosis of these infections is crucial for effective treatment and patient outcomes. CLSI M22 plays a critical role in ensuring that blood cultures are collected, transported, and processed in a way that maximizes their diagnostic yield. By following the guidelines outlined in CLSI M22, healthcare professionals can help reduce the risk of contamination, false-negative results, and inappropriate antimicrobial therapy. clsi m22 pdf