This is why special tools are required to "burn" or "write" an ISO to a USB drive or DVD properly.
Programs like VirtualBox or VMware can directly load an ISO as a virtual DVD, letting you test Windows without affecting your main system. what is windows iso file
For users running Virtual Machines (VMs) using software like VirtualBox or VMware, the ISO is the standard input method. It allows the virtual computer to "mount" the ISO as if it were a real CD-ROM drive to install the OS. This is why special tools are required to